Downregulation of DNA topoisomerase IIalpha leads to prolonged cell cycle transit in G2 and early M and increased survival to microtubule-interacting agents
نویسندگان
چکیده
Group of Molecular and Clinical Cancer Therapeutics, INSERM U673, Hôpital Saint-Antoine, Paris, France (A.S., M.G.C., A.E.E., A.K.L.) and Laboratory of Molecular and Cellular Pharmacology, Department of Pharmaceutical Technology and Biochemistry, Gdansk University of Technology, Gdansk, Poland (A.S., M.S.) Molecular Pharmacology Fast Forward. Published on June 7, 2005 as doi:10.1124/mol.105.013995
منابع مشابه
Down-regulation of DNA topoisomerase IIalpha leads to prolonged cell cycle transit in G2 and early M phases and increased survival to microtubule-interacting agents.
Microtubule binders are cell cycle-specific agents with preferential cytotoxicity toward mitotic cells. We have characterized vincristine-selected human leukemia cells to establish whether development of vincristine resistance was accompanied by changes in cell cycle kinetics and distribution. Our results indicate that vincristine resistance is accompanied by delayed G2 transit and prolonged ea...
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We related cellular content of DNA topoisomerase (topo) IIalpha and IIbeta with the cell cycle position in proliferating, differentiated, and apoptotic HL-60 cells using two-dimensional flow cytometry. In logarithmically growing HL-60 cells, topo IIalpha increased especially in late S to G2/M phases, although the topo IIbeta level was almost constant throughout the cell cycle. Induction of diff...
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We here report the influence of the cell cycle abrogator UCN-01 on RKO human colon carcinoma cells differing in p53 status following exposure to two DNA damaging agents, the topoisomerase inhibitors etoposide and camptothecin. Cells were treated with the two drugs at the IC90 concentration for 24 h followed by post-incubation in drug-free medium. RKO cells expressing wild-type, functional p53 a...
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تاریخ انتشار 2005